Welcome to The Home Page for The Salem Laboratory of Advanced Drug and Gene Delivery. Dr. Salem's research interests are primarily focused on self-assembling systems, the rational design of novel drug and gene delivery systems and on the development of vaccines that stimulate potent antigen-specific immune responses. Dr Salem's laboratory applies microfabrication techniques to develop novel drug and gene delivery devices and to optimize control over polymer-cell interactions. The group is currently exploring the synergistic application of polymer particle technology, CpG oligonucleotides, adenoviruses and heat shock protein therapy for generating sustained stronger immune responses against tumors. The group is also working on new materials based methods and approaches for enhancing tissue regeneration.
Selected Publication Highlights:
R. Smith†, E.I. Wafa†, S.M. Geary†, K. Ebeid, S.O. Alhaj-Suliman, A.K. Salem. Cationic nanoparticles enhance T-cell tumor infiltration and antitumor immune response to a melanoma vaccine. Science Advances. 2022.
K. Ebeid, X. Meng, K.W. Thiel, A. Do, S.M. Geary, A. S. Morris, E.L. Pham, A. Wongrakpanich, Y.S. Chhonker, D.J. Murry, K.K. Leslie, A.K. Salem. Synthetically Lethal Nanoparticles for Treatment of Endometrial Cancer. Nature Nanotechnology. 2018.
M.C. Coleman, J.E. Goetz, M.J. Brouillette, D. Seol, M.C. Willey, E.B. Petersen, B. Khorsand, A.S. Morris, A.K. Salem, D.C. Fredericks, T.O. McKinley, J.A. Martin. Mitochondrial Responses to Intraarticular Fracture are a Disease-Modifying Target for Posttraumatic Osteoarthritis Prevention. Science Translational Medicine. 2018.
B. Khorsand, N. Nicholson, A. Do, J.E. Femino, J.A. Martin, E. Petersen, B. Guetschow, D.C. Fredericks, A.K. Salem. Regeneration of bone using synergistic FGF2 and BMP2 gene delivery in a diaphyseal long bone radial defect diabetic rabbit model. Journal of Controlled Release. 2017.
K.K. Ahmed, S.M. Geary, and A.K. Salem. Surface engineering tumor cells with adjuvant-loaded particles for use as cancer vaccines. Journal of Controlled Release. 2017.
K.S. Worthington, B.J. Green, M. Rethwisch, L.A. Wiley, B.A. Tucker, and C.A.Guymon and A.K. Salem. Neuronal Differentiation of Induced Pluripotent Stem Cells on Surfactant Templated Chitosan Hydrogels. Biomacromolecules. 2016.
S. Elangovan*, B. Khorsand*, A. Do, L. Hong, A. Dewerth, M. Kormann, R.D. Ross, D.R. Sumner, C. Allamargot, and A.K. Salem. Chemically modified RNA activated matrix induced bone regeneration. Journal of Controlled Release. 2015. *Joint First Authors
S.M. Geary, Q. Hu, V.B. Joshi, N.B. Bowden, A.K. Salem. Diaminosulfide based polymer microparticles as cancer vaccine delivery systems. Journal of Controlled Release. 2015
D. Rajasekaran#, J. Srivastava#, K. Ebeid#, R. Gredler, M. Akiel, N. Jariwala, C.L. Robertson, X. Shen, A. Siddiq, P.B. Fisher, A.K. Salem*, Devanand Sarkar*. Combination of nanoparticle-delivered siRNA for Astrocyte elevated gene-1 (AEG-1) and all-trans retinoic acid (ATRA): an effective therapeutic strategy for hepatocellular carcinoma (HCC). Bioconjugate Chemistry. #Joint First Authors *Corresponding Authors. 2015
S. Elangovan, S.R. D’Mello, L. Hong, R.D. Ross, C. Allamargot, D.V. Dawson, C.M. Stanford, G.K. Johnson, D.R. Sumner, and A.K. Salem. The Enhancement of Bone Regeneration by Gene Activated Matrix Encoding for Platelet Derived Growth Factor. Biomaterials. 2014.
S.M. Geary, C.D. Lemke, D.M. Lubaroff and A.K. Salem. Proposed mechanisms of action for prostate cancer vaccines. Nature Reviews Urology. 2013.
C.D. Lemke, S.M. Geary, V.B. Joshi, A.K. Salem. Antigen coated poly α-hydroxy acid based microparticles for heterologous prime-boost adenovirus based vaccinations. Biomaterials. 2013.
V.B. Joshi, S.M. Geary, A.K. Salem. Biodegradable particles as vaccine delivery systems: Size matters. The AAPS Journal. 2013.
J. Intra, A.K. Salem. Characterization of the transgene expression generated by branched and linear polyethylenimine (PEI)-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo. Journal of Controlled Release (130) 129-1382008.
J. Intra, J.M Glasgow, H.Q. Mai, A.K. Salem. Pulsatile release of biomolecules from polydimethylsiloxane (PDMS) chips with hydrolytically degradable seals. Journal of Controlled Release (127) 280-287 2008.
J.D. Clapper, M.E. Pearce, C.A. Guymon, A.K. Salem. Biotinylated biodegradable nanotemplated hydrogel networks for cell interactive applications. Biomacromolecules (9) 1188-1194 2008.
Y. Krishnamachari, M.E. Pearce, A.K. Salem. Self-assembly of cell-microparticle hybrids. Advanced Materials. 20 (5): 989-993 Mar 2008.
X.Q. Zhang, J. Intra, A.K. Salem. Conjugation of polyamidoamine dendrimers on biodegradable microparticles for non-viral gene delivery. Bioconjugate Chemistry. 2007 Nov-Dec;18 (6):2068-76.
X.Q. Zhang, C.E. Dahle, N.K. Baman, N. Rich, G.J. Weiner and A.K. Salem. Potent antigen-specific immune responses stimulated by co-delivery of CpG ODN and antigens in degradable microparticles. Journal of Immunotherapy, 30 (5): 469-478 July/August
J. Sinclair, A.K. Salem. Rapid localized cell trapping on biodegradable polymers using cell surface derivatization and microfluidic networking. Biomaterials 27 2090-2094 MAR 2006.
A.K. Salem, M. Chen, J. Hayden, K.W. Leong, and P.C. Searson. Directed assembly of multi-segment Au/Pt/Au nanowires. Nanoletters.4 (6): 1163-1165 JUN 2004.
A.K. Salem. J. Chao. K.W. Leong, and P.C. Searson. Receptor mediated self-assembly of multi-component magnetic nanowires. Advanced Materials 16 (3): 268+ FEB 3 2004.
A.K. Salem, P.C. Searson and K.W. Leong. Multifunctional nanorods for gene delivery. Nature Materials. Oct 2003, 2, 668-671.
H.S. Sakhalkar, M.K. Dalal, A.K. Salem, R. Ansari, J. Fu, M.F. Kiani, J. Hanes, K.M. Shakesheff and D.J. Goetz. Leukocyte inspired therapeutic particles that selectively and avidly adhere to inflamed endothelium. Proceedings of the National Academy of Sciences.100 (26): 15895-15900 DEC 23 2003.
A.K. Salem, F.R.A.J. Rose, R. Oreffo, X. Yang, M.C. Davies, J.R. Mitchell, C.J. Roberts, S. Stolnik, S.J.B. Tendler, P.M. Williams and K.M. Shakesheff. Porous polymer and cell composites that self assemble. Advanced Materials. 15 (3): 210-213 FEB 5 2003.